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Abstract The National Science Foundation–sponsored Lake-Effect Electrification (LEE) field campaign intensive observation periods occurred between November and early February 2022–23 across the eastern Lake Ontario region. Project LEE documented, for the first time, the total lightning and electrical charge structures of lake-effect storms and the associated storm environment using a lightning mapping array (LMA), a mobile dual-polarization X-band radar, and balloon-based soundings that measured vertical profiles of temperature, humidity, wind, electric field, and hydrometeor types. LEE also observed abundant wind turbine-initiated lightning, which is climatologically more likely during the winter. The frequent occurrence of intense lake-effect storms and the proximity of a wind farm with nearly 300 turbines each more than 100 m tall to the lee of Lake Ontario provided an ideal laboratory for this study. The field project involved many undergraduate (>20) and graduate students. Some foreseen and unforeseen challenges included clearing the LMA solar panels of snow and continuous operation in low-sunlight conditions, large sonde balloons prematurely popping due to extremely cold conditions, sonde line breaking, recovering probes in deep snow in heavily forested areas, vehicles getting stuck in the snowpack, and an abnormally dry season for parts of the LEE domain. In spite of these difficulties, a dataset was collected in multiple lake-effect snowstorms (11 observation periods) and one extratropical cyclone snowstorm that clarifies the electrical structure of these systems. A key finding was the existence of a near-surface substantial positive charge layer (1 nC m⁻³) near the shoreline during lake-effect thunderstorms. 

In this paper, we discuss some of the key challenges in the study of time-dependent processes and non-equilibrium behaviour in warm dense matter. We outline some of the basic physics concepts that have underpinned the definition of warm dense matter as a subject area in its own right and then cover, in a selective, non-comprehensive manner, some of the current challenges, pointing along the way to topics covered by the papers presented in this volume. This article is part of the theme issue ‘Dynamic and transient processes in warm dense matter’. 

Warm dense matter is a material state in the region of parameter space connecting condensed matter to classical plasma physics. In this intermediate regime, we investigate the significance of non-adiabatic electron-ion interactions upon ion dynamics. To disentangle non-adiabatic from adiabatic electron-ion interactions, we compare the ion self-diffusion coefficient from the non-adiabatic electron force field computational model with an adiabatic, classical molecular dynamics simulation. A classical pair potential developed through a force-matching algorithm ensures the only difference between the models is due to the electronic inertia. We implement this new method to characterize non-adiabatic effects on the self-diffusion of warm dense hydrogen over a wide range of temperatures and densities. Ultimately we show that the impact of non-adiabatic effects is negligible for equilibrium ion dynamics in warm dense hydrogen. This article is part of the theme issue ‘Dynamic and transient processes in warm dense matter’. 

Serial femtosecond crystallography (SFX) with X-ray free-electron lasers (XFELs) has proven highly successful for structure determination of challenging membrane proteins crystallized in lipidic cubic phase; however, like most techniques, it has limitations. Here we attempt to address some of these limitations related to the use of a vacuum chamber and the need for attenuation of the XFEL beam, in order to further improve the efficiency of this method. Using an optimized SFX experimental setup in a helium atmosphere, the room-temperature structure of the adenosine A_2Areceptor (A_2AAR) at 2.0 Å resolution is determined and compared with previous A_2AAR structures determined in vacuum and/or at cryogenic temperatures. Specifically, the capability of utilizing high XFEL beam transmissions is demonstrated, in conjunction with a high dynamic range detector, to collect high-resolution SFX data while reducing crystalline material consumption and shortening the collection time required for a complete dataset. The experimental setup presented herein can be applied to future SFX applications for protein nanocrystal samples to aid in structure-based discovery efforts of therapeutic targets that are difficult to crystallize. 

Abstract Time-resolved studies of biomacromolecular crystals have been limited to systems involving only minute conformational changes within the same lattice. Ligand-induced changes greater than several angstroms, however, are likely to result in solid-solid phase transitions, which require a detailed understanding of the mechanistic interplay between conformational and lattice transitions. Here we report the synchronous behavior of the adenine riboswitch aptamer RNA in crystal during ligand-triggered isothermal phase transitions. Direct visualization using polarized video microscopy and atomic force microscopy shows that the RNA molecules undergo cooperative rearrangements that maintain lattice order, whose cell parameters change distinctly as a function of time. The bulk lattice order throughout the transition is further supported by time-resolved diffraction data from crystals using an X-ray free electron laser. The synchronous molecular rearrangements in crystal provide the physical basis for studying large conformational changes using time-resolved crystallography and micro/nanocrystals.